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June 24, 2020


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What is plant tissue culture?

What is plant tissue culture?

Plant tissue culture is a group of techniques used to sustain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. Plant tissue culture is widely used to produce clones of a plant in a method known as micro propagation.


Reference: James Sigman

Why do plants grow faster in tissue culture?

Why do plants grow faster in tissue culture?

  • Plants do not grow 'fast' in tissue culture as such.  As a matter of fact photosynthates (sucrose etc) have to be added to the medium to sustain plant growth as a matter of routine.
  • However some morphogenetic activities can be made fast on a micro scale and used skillfully:
  • Firstly on a high auxin:ctokinin medium explant cells divide rapidly to produce "callus".  This can be grown and multiplied.  At a give point of time it can be shifted to a medium with high cytokinin.  It is then it rapidly  differentiates many shoots.  The shoots are made to root and have to be shifted to pots out in a green house for fast growth.
  • Likewise a small shoot can be planted on a medium with high cytokinin.  Here it shall quickly  produce "multiple axillary"shoots.  These again must be skillfully excised, rooted and shifted to screen house pots appropriately.
Reference: Rajiv Angrish, Professor Plant Physiology

What are the differences between human cancer and plant cancer?

What are the differences between human cancer and plant cancer?

Plants develop tumor like undifferentiated mass of tissue called callus tissue which is similar to cancerous tissue in animals. Depending on the plant hormones, auxins and cytokinins concentration, differentiation into roots or shoots take place. However, when the concentration of both these hormones are high, callus formation is noted in plant tissue culture.

Reference: Pranab Roy, Head, Molecular Biology at Institute of Child Health, Kolkata (2018-present)

How is tissue culture done?

How is tissue culture done?

This process involves the use of small pieces of a given plant tissue (plant of interest). Once the tissue is obtained, it is then cultured in the appropriate medium under sterile conditions so as to prevent various types of microorganisms from affecting the process.

Reference: Bhanita Sarmah, Masters Tourism & Travel, Indira Gandhi National Open University (2019)

What is a phytoplasma?

What is a phytoplasma?

Phytoplasmas are obligate bacterial parasites of plant phloem tissue and of the insect vectors that are involved in their plant-to-plant transmission. Phytoplasmas were discovered in 1967 by Japanese scientists who termed them mycoplasma-like organisms.

Since their discovery, phytoplasmas have resisted all attempts at in vitro culture in any cell-free medium; routine cultivation in an artificial medium thus remains a major challenge. Although phytoplasmas have recently been reported to be grown in a specific artificial medium, experimental repetition has yet to be reported.

Phytoplasmas are characterized by the lack of a cell wall, a pleiomorphic or filamentous shape, a diameter normally less than 1 ?m, and a very small genome.

Reference: Bhanita Sarmah, Masters Tourism & Travel, Indira Gandhi National Open University (2019) (Quora)

Why is China affected by the coronavirus?

Why is China affected by the coronavirus?

Probably an attempted biological attack, since China would be the best place to plant the virus, since the Chinese citizens travel around the world a lot, whether it be for sightseeing, visiting overseas relatives, or big businesses all around the world.

Reference: Tony Dusk, Ancient Hero from Cradle of Mankind (1900) (Quora)